Spectrophotometry is an analytical, qualitative and quantitative technique which allows recognition and quantization of a substance based on its absorption spectrum of light. The spectrophotometric analysis is applicable, with certain expedients, to all substances; if the test substance is itself light-absorbing at the wavelength choice is possible a direct measurement, if it is not then it can participate in a chemical reaction in which the substance itself or an its derivative reacts with a dye giving a colored end product. The UV-Vis spectrophotometer consists of the following components:

  • a light source which gives rise to a polychromatic radiation in a certain range of wavelengths (tungsten filament lamp for visible light, deuterium lamp for ultraviolet radiation)
  • a wavelength selector or monochromator which solves the polychromatic beam in radiations of a determined wavelength
  • an holder for the introduction of the cuvette
  • a photoelectric cell on which affects the beam emerging from the testing solution;
  • an amplification system of the electric current emitted by the photocell
  • a galvanometer for measuring the electric curren.



Concentration measurements, Determination of enzyme kinetics, Absorption spectra to identify chromophore groups and molecules, Measurement of color. 



Ultraviolet-visible spectrophotometry.



Additional Info

  • Wavelength range: 190 ÷ 900 nm
  • Light source: 190 ÷ 350 nm
  • Light source: halogen lamp: 330 ÷ 900 nm
  • Bandwidth (or resolution): 0,1 nm
  • Location: Chemistry Laboratory - ProdAl
More in this category: « Colorimeter TEMPO system »

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